Figure 8. Effect of PPARγ and FASN overexpression on the viability of hypoxia-induced cardiomyocytes. Isolated cardiomyocytes were transfected with pcDNA3-NC, pcDNA3-PPARγ, and pcDNA3-FASN for 24 h followed by hypoxia exposure for 24 h. Cells under normoxia served as controls. (A) The CCK-8 assay revealed the cell viability in each group. (B) Annexin V-FITC/PI flow cytometry was utilized to detect the proportion of apoptotic cardiomyocytes (n = 3). *P < 0.05 and **P < 0.01 vs. the normoxia group. ##P < 0.01 vs. the hypoxia group.