Research Paper Volume 13, Issue 10 pp 13535—13547

FAM134B-mediated endoplasmic reticulum autophagy protects against sepsis myocardial injury in mice

FAM134B reduced the inflammatory response and regulated the autophagy-related protein expressions of sepsis myocardial injury in mice. (A) The levels of TNF-α, IL-6, IL-8, and IL-10 were evaluated by ELISA. (B, C) The mRNA and protein expressions of LC3-II/I, IRE1α, GRP78, Beclin-1, and LAMP2 in the myocardial tissues of four groups (Sham, Sep, Sep+Rap+siFAM134B, and Sep+3-MA+oeFAM134B) were detected by RT-PCR and western blot, respectively. Data are shown as mean ± SD. *P

Figure 3. FAM134B reduced the inflammatory response and regulated the autophagy-related protein expressions of sepsis myocardial injury in mice. (A) The levels of TNF-α, IL-6, IL-8, and IL-10 were evaluated by ELISA. (B, C) The mRNA and protein expressions of LC3-II/I, IRE1α, GRP78, Beclin-1, and LAMP2 in the myocardial tissues of four groups (Sham, Sep, Sep+Rap+siFAM134B, and Sep+3-MA+oeFAM134B) were detected by RT-PCR and western blot, respectively. Data are shown as mean ± SD. *P<0.05, **P<0.01 and ***P<0.001. Sep: Sepsis. SD: standard deviation.