Research Paper Volume 13, Issue 10 pp 13535—13547

FAM134B-mediated endoplasmic reticulum autophagy protects against sepsis myocardial injury in mice

The effect of FAM134B on LPS-treated mouse cardiomyocytes. (A) Cardiomyocytes were transfected with oeNC, oeFAM143B, siNC, siFAM134B, and the mRNA and protein expressions of FAM134B were detected by RT-PCR and western blot analysis. (B) Cardiomyocytes with transfection were then treated with LPS, and cell proliferation was identified by CCK8 assay. (C) Cell apoptosis was examined by Annexin V/PI staining. (D) The release of inflammatory factors was evaluated by ELISA. Data are shown as mean ± SD. *P

Figure 5. The effect of FAM134B on LPS-treated mouse cardiomyocytes. (A) Cardiomyocytes were transfected with oeNC, oeFAM143B, siNC, siFAM134B, and the mRNA and protein expressions of FAM134B were detected by RT-PCR and western blot analysis. (B) Cardiomyocytes with transfection were then treated with LPS, and cell proliferation was identified by CCK8 assay. (C) Cell apoptosis was examined by Annexin V/PI staining. (D) The release of inflammatory factors was evaluated by ELISA. Data are shown as mean ± SD. *P<0.05, **P<0.01 and ***P<0.001. SD: standard deviation.