Research Paper Volume 13, Issue 7 pp 10240—10274

Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut–brain axis

Influences of maternal high sugar and fat diet on autophagy and neurodevelopment of offspring during the growth stage. (A) Changes on the body weight; (B) Changes on inflammatory factors in serum; (C) the marked proteins expression of KGA/GAD, MAP2, GFAP, Vimentin, IBA1, GAD65, DYNC1H1, Myclin, Tubulin, CHAT, OLIG2 and WFS1 in brain tissues, (D) and the expression LC3A I/LC3A II/ LC3B I/LC3B II protein in brain tissues. The symbol of NA is the 16-month control male samples, NB is the 16-month control female samples; MA is the 16-month HSHF male samples, MB is the 16-month HSHF female samples. Data are presented as the means ± SD of more than 8 independent experiments, and more than 3 independent experiments in Western bolting. *p **p vs. the model group by one-way ANOVA, followed by the one-way analysis of variance (ANOVA) at p

Figure 2. Influences of maternal high sugar and fat diet on autophagy and neurodevelopment of offspring during the growth stage. (A) Changes on the body weight; (B) Changes on inflammatory factors in serum; (C) the marked proteins expression of KGA/GAD, MAP2, GFAP, Vimentin, IBA1, GAD65, DYNC1H1, Myclin, Tubulin, CHAT, OLIG2 and WFS1 in brain tissues, (D) and the expression LC3A I/LC3A II/ LC3B I/LC3B II protein in brain tissues. The symbol of NA is the 16-month control male samples, NB is the 16-month control female samples; MA is the 16-month HSHF male samples, MB is the 16-month HSHF female samples. Data are presented as the means ± SD of more than 8 independent experiments, and more than 3 independent experiments in Western bolting. *p <0.05 and **p <0.01 vs. the model group by one-way ANOVA, followed by the one-way analysis of variance (ANOVA) at p < 0.05.