Research Paper Volume 13, Issue 5 pp 6485—6505

Clusterin overexpression in mice exacerbates diabetic phenotypes but suppresses tumor progression in a mouse melanoma model

CLU is overexpressed in tissues of the TgN102 and TgG106 (ubiquitous CLU OE) mice. (A) Relative clu mRNA expression levels in the heart, muscle, brain, liver, pancreas, lung, intestine, and colon of TgN102, TgG106 lines and control (littermate non-Tg) animals. (B) Representative immunoblot analyses in shown tissue samples [whole cell lysates and cell membranes (pellets)] from Tg or control animals probed with a CLU antibody; GAPDH probing was used as a reference. (C) Immunoblot analyses of CLU expression levels in serum of shown Tg or control animals; IgG probing was used as loading reference. Error bars, ± SD (n=4 per mouse genotype); *PP

Figure 1. CLU is overexpressed in tissues of the TgN102 and TgG106 (ubiquitous CLU OE) mice. (A) Relative clu mRNA expression levels in the heart, muscle, brain, liver, pancreas, lung, intestine, and colon of TgN102, TgG106 lines and control (littermate non-Tg) animals. (B) Representative immunoblot analyses in shown tissue samples [whole cell lysates and cell membranes (pellets)] from Tg or control animals probed with a CLU antibody; GAPDH probing was used as a reference. (C) Immunoblot analyses of CLU expression levels in serum of shown Tg or control animals; IgG probing was used as loading reference. Error bars, ± SD (n=4 per mouse genotype); *P<0.05; **P<0.01.