Research Paper Volume 13, Issue 7 pp 10312—10325

Membrane-cytoplasm translocation of annexin A4 is involved in the metastasis of colorectal carcinoma

Calcium regulated the expression and location of ANXA4. (A) Time-course expression analysis of ANXA4 after the addition of the Ca2+ agonist ionomycin (1 μM or 2 μM) at 14 h and 24 h. Tubulin was used as the loading control for total protein. *p B) Time-course expression analysis of CAMK2γ after the addition of the Ca2+ agonist ionomycin (1 μM or 2 μM) at 14 h and 24 h. Tubulin was used as the loading control for total protein. *p C) Time-course expression analysis of ANXA4 in HT-29 cells transfected with CAMK2γ after the addition of the Ca2+ agonist ionomycin (1 μM or 2 μM) at 14 h and 24 h. Tubulin was used as the loading control for total protein. *p D) Membrane and cytoplasmic levels of ANXA4 in HT-29 cells transfected with CAMK2γ after the addition of the Ca2+ agonist ionomycin (1 μM) at 14 h. Na/K-ATPase was used as the loading control for cell membrane protein; Tubulin was used as the loading control for total protein. *p E–H) Relative quantitative of comparison of WB analysis mentioned in (A–D). *p

Figure 2. Calcium regulated the expression and location of ANXA4. (A) Time-course expression analysis of ANXA4 after the addition of the Ca2+ agonist ionomycin (1 μM or 2 μM) at 14 h and 24 h. Tubulin was used as the loading control for total protein. *p < 0.05; **p < 0.01; **p < 0.001. (B) Time-course expression analysis of CAMK2γ after the addition of the Ca2+ agonist ionomycin (1 μM or 2 μM) at 14 h and 24 h. Tubulin was used as the loading control for total protein. *p < 0.05; **p < 0.01; **p < 0.001. (C) Time-course expression analysis of ANXA4 in HT-29 cells transfected with CAMK2γ after the addition of the Ca2+ agonist ionomycin (1 μM or 2 μM) at 14 h and 24 h. Tubulin was used as the loading control for total protein. *p < 0.05; **p < 0.01; ***p < 0.001. (D) Membrane and cytoplasmic levels of ANXA4 in HT-29 cells transfected with CAMK2γ after the addition of the Ca2+ agonist ionomycin (1 μM) at 14 h. Na/K-ATPase was used as the loading control for cell membrane protein; Tubulin was used as the loading control for total protein. *p < 0.05; **p < 0.01; ***p < 0.001. (EH) Relative quantitative of comparison of WB analysis mentioned in (AD). *p < 0.05; **p < 0.01; ***p < 0.001.