Ischemic-hypoxic preconditioning enhances the mitochondrial function recovery of transplanted olfactory mucosa mesenchymal stem cells via miR-181a signaling in ischemic stroke

Yi Zhuo; Wei Chen; Wenshui Li; Yan Huang; Da Duan; Lite Ge; Jialin He; Jianyang Liu; Zhiping Hu; Ming Lu

doi:doi:10.18632/aging.202807

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Research Paper Volume 13, Issue 8 pp 11234—11256

Ischemic-hypoxic preconditioning enhances the mitochondrial function recovery of transplanted olfactory mucosa mesenchymal stem cells via miR-181a signaling in ischemic stroke

Figure 2. Determination of optimal cultivation scheme of IhOM-MSCs. (A) We were pretreated OM-MSCs with ischemia/hypoxia at different time gradients, and the RT-qPCR result showed that miR-181a was significantly down-regulated after 36h. (B) Successful mimic of miR-181a in IhOM-MSCs was verified by qPCR. (C–E) Protein and mRNA expression of GRP78 and Bcl-2 in OM-MSCs as determined by western blot and qPCR, respectively. (OM-MSCs were replaced by MSCs in the figure. All data are presented as the mean value ± SD. *p<0.05, **p<0.01).