Figure 2. Chondrogenic effects of FRZB on BMSCs in vitro. (A) Alcian blue staining of BMSCs in different treatment groups at 2 weeks to indicate GAG production in the culture plate. (B) Quantification of GAG production in generated cartilaginous tissues (n=6 for each). (C) Immunofluorescent assay of Aggrecan (green) expression and nucleus (blue) in different treatment groups observed under confocal microscopy. (D–F) Expression level of chondrogenic markers for BMSCs in different treatment groups (n=6 for each) in (A) (n=6 for each). *P < 0.05 between control group and other groups. Data are presented as averages ± SD. One-way analysis of variance (ANOVA) with post-hoc Tukey’s B test was applied. Ab, antibody; Exo, exogenous; ACAN, aggrecan; GAG, glycosaminoglycan.