Research Paper Volume 13, Issue 8 pp 11281—11295

Secreted frizzled-related protein 3 was genetically and functionally associated with developmental dysplasia of the hip

FRZB modulated cell adhesion pathway and cell spreading by regulating integrins expressions. (A) Protein-protein interaction with enriched cell adhesion molecule and focal adhesion signaling pathway-related genes. Integrin family members ITGA8 and ITGAV (red arrow) were enriched in both pathways. (B) Western blot experiment validated ITGA8 and ITGAV expression in FRZB-siRNA group. (C) Gene expressions of cell adhesion pathway genes were also validated and quantified with RT-PCR. (D, E) Greater cell spreading was demonstrated in FRZB-knockdown cells with cytoskeleton staining (green: phalloidin, blue: nucleus). (F, H) Effects of exogenous FRZB or FRZB blockage on cell spreading and (G) its regulation of related genes in cell adhesion signaling pathway.

Figure 5. FRZB modulated cell adhesion pathway and cell spreading by regulating integrins expressions. (A) Protein-protein interaction with enriched cell adhesion molecule and focal adhesion signaling pathway-related genes. Integrin family members ITGA8 and ITGAV (red arrow) were enriched in both pathways. (B) Western blot experiment validated ITGA8 and ITGAV expression in FRZB-siRNA group. (C) Gene expressions of cell adhesion pathway genes were also validated and quantified with RT-PCR. (D, E) Greater cell spreading was demonstrated in FRZB-knockdown cells with cytoskeleton staining (green: phalloidin, blue: nucleus). (F, H) Effects of exogenous FRZB or FRZB blockage on cell spreading and (G) its regulation of related genes in cell adhesion signaling pathway.