Figure 5. FRZB modulated cell adhesion pathway and cell spreading by regulating integrins expressions. (A) Protein-protein interaction with enriched cell adhesion molecule and focal adhesion signaling pathway-related genes. Integrin family members ITGA8 and ITGAV (red arrow) were enriched in both pathways. (B) Western blot experiment validated ITGA8 and ITGAV expression in FRZB-siRNA group. (C) Gene expressions of cell adhesion pathway genes were also validated and quantified with RT-PCR. (D, E) Greater cell spreading was demonstrated in FRZB-knockdown cells with cytoskeleton staining (green: phalloidin, blue: nucleus). (F, H) Effects of exogenous FRZB or FRZB blockage on cell spreading and (G) its regulation of related genes in cell adhesion signaling pathway.