Figure 2. Hepcidin overexpression has little effect on osteoblasts in the OVX mouse. (A, B) Hepcidin overexpression decrease the irons contents in the mouse bone. (C, D) ALP stain shows that overexpression Hepcidin does not significantly inhibit the number of osteoblasts in femoral bone in the OVX mouse; (E, F) Osteoblasts, which cultured by mouse serum for 14 or 21 days, stained with ALP or alizarin red to assess its differentiation and mineralization; (G, H) Quantitative polymerase chain reaction (q-PCR) analysis of the expression of bone formation markers including Alp and Bmp2. Scale bar, 200 μm. The asterisks (*) indicate significant differences at P < 0.05.