Research Paper Volume 13, Issue 7 pp 10584—10602

Suppression of choroidal neovascularization by silencing of long non-coding RNA IPW

LncRNA-IPW/miR-370 interaction regulates the development of CNV in vivo and ex vivo. (A and B) The mice were injected with scrambled (Scr) agomir, miR-370 agomir, miR-370 agomir plus AAV vector, miR-370 agomir plus AAV-IPW, or left untreated (Ctrl). On day 14 after laser photocoagulation, the mice were euthanized and RPE/choroid complexes were dissected and flat-mounted. The blood vessels were stained with Isolectin-B4 (n = 6 animals/group; *P #P C and D) The mice received an intravitreal injection of Scr agomir, miR-370 agomir, miR-370 agomir plus Vector, miR-370 agomir plus IPW, or left untreated (Ctrl). On day 14 after laser photocoagulation, the RPE/choroid complexes were dissected and choroidal explants were performed as previously described. The representative images of choroidal explants after 4-day, 5-day, and 6-day culture were shown (C). Scale bar: 500 μm. Meanwhile, the quantification results of sprouting areas were shown (D; n = 6 animals/group; *P #P

Figure 7. LncRNA-IPW/miR-370 interaction regulates the development of CNV in vivo and ex vivo. (A and B) The mice were injected with scrambled (Scr) agomir, miR-370 agomir, miR-370 agomir plus AAV vector, miR-370 agomir plus AAV-IPW, or left untreated (Ctrl). On day 14 after laser photocoagulation, the mice were euthanized and RPE/choroid complexes were dissected and flat-mounted. The blood vessels were stained with Isolectin-B4 (n = 6 animals/group; *P < 0.05 versus Ctrl group; #P < 0.05 miR-370 agomir group versus miR-370 agomir plus IPW group; Kruskal-Wallis test). The representative images of Isolectin-B4-stained flat-mounted choroid on day 14 after laser induction. Dashed lines delineate lesion areas. Scale bar: 200 μm (B). (C and D) The mice received an intravitreal injection of Scr agomir, miR-370 agomir, miR-370 agomir plus Vector, miR-370 agomir plus IPW, or left untreated (Ctrl). On day 14 after laser photocoagulation, the RPE/choroid complexes were dissected and choroidal explants were performed as previously described. The representative images of choroidal explants after 4-day, 5-day, and 6-day culture were shown (C). Scale bar: 500 μm. Meanwhile, the quantification results of sprouting areas were shown (D; n = 6 animals/group; *P < 0.05 miR-370 agomir versus Ctrl group; #P < 0.05 miR-370 agomir + IPW OE versus miR-370 agomir; Kruskal-Wallis test). Scale bar: 1 mm.