Research Paper Volume 13, Issue 8 pp 11363—11380

Melatonin attenuates smoking-induced atherosclerosis by activating the Nrf2 pathway via NLRP3 inflammasomes in endothelial cells

Nrf2 negatively regulated CSE-induced NLRP3 inflammasome activation. (A–C) The mRNA level of NLRP3, IL-1β and IL-18 was detected by RT-PCR. (D–K) NLRP3, Pro-Caspase-1, Caspase-1, Pro-IL-1β, IL-1β, Pro-IL-18 and IL-18 were detected by Western blot. *p p ns, not significant, compared with CSE group. The data are represented as mean ± SD (n = 3).

Figure 5. Nrf2 negatively regulated CSE-induced NLRP3 inflammasome activation. (AC) The mRNA level of NLRP3, IL-1β and IL-18 was detected by RT-PCR. (DK) NLRP3, Pro-Caspase-1, Caspase-1, Pro-IL-1β, IL-1β, Pro-IL-18 and IL-18 were detected by Western blot. *p < 0.05, **p < 0.01, ns, not significant, compared with CSE group. The data are represented as mean ± SD (n = 3).