Figure 9. TGLQ reduced the accumulation of lipid in ox-LDL induced macrophages. (A) Oil red O Staining was applied to estimate the level of lipid accumulation; (B–D) represent the expression levels of total cholesterol (TC), free cholesterol (FC) and cholesterol ester (CE) in each group respectively. The levels of TC, FC and CE in the culture medium were detected by using commercial kits. (E) Percentage of TC to CE ratio in each group. Unlike the macrophages of the control group, the macrophages of the model group, the low-dose TGLQ-serum (LTGLQ) group and the high-dose TGLQ-serum (HTGLQ) group were exposed to 60μg/ml oxidized-LDL (ox-LDL) for 24 h. In addition, control serum, 5% TGLQ serum and 20% TGLQ serum were added to the model group, the LTGLQ group and the HTGLQ group, respectively. #, ##, and ### represent P < 0.05, P < 0.01, and P < 0.001 respectively, in comparison with the control group. *, **, and *** represent P < 0.05, P < 0.01, and P < 0.001 respectively, in comparison with the model group. %, %% and %%% represent P < 0.05, P < 0.01 and P < 0.001 respectively, in comparison with the LTGLQ group. n.s represents P > 0.05.