Research Paper Volume 13, Issue 8 pp 11433—11454

Overexpression of long non-coding RNA AP001505.9 inhibits human hyaline chondrocyte dedifferentiation

Seven days of pellet culture induces redifferentiation of P5 chondrocytes. (A) Hematoxylin-eosin (HE) staining results. (B) Safranin O staining. (C) Alcian staining. (D) Immunohistochemistry for COL2. (E) Immunohistochemistry for SOX-9. (F) Immunohistochemistry for COL1. (G) Real-time qPCR for the cartilage-related marker genes COL1A1, COL2A1, and SOX-9. (H, I) Real-time qPCR for the lncRNAs AP001505.9, LINC00162, LINC01021, and GS1-600G8.5. GAPDH was used as the internal reference. Data are represented as means ± standard deviation. *P P P

Figure 4. Seven days of pellet culture induces redifferentiation of P5 chondrocytes. (A) Hematoxylin-eosin (HE) staining results. (B) Safranin O staining. (C) Alcian staining. (D) Immunohistochemistry for COL2. (E) Immunohistochemistry for SOX-9. (F) Immunohistochemistry for COL1. (G) Real-time qPCR for the cartilage-related marker genes COL1A1, COL2A1, and SOX-9. (H, I) Real-time qPCR for the lncRNAs AP001505.9, LINC00162, LINC01021, and GS1-600G8.5. GAPDH was used as the internal reference. Data are represented as means ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bar, 50 μm.