Agomelatine restored the expression of claudin-5 in hypoxic endothelial cells. Brain bEnd.3 endothelial cells were incubated with 10 μM Agomelatine in the process of hypoxia/ reperfusion (H/R). (A). mRNA of claudin-5 as measured by real-time PCR; (B). Protein expression of claudin-5 as measured by western blot analysis (****P < 0.0001 vs. vehicle group; ####P < 0.0001 vs. H/R group).