Research Paper Volume 13, Issue 8 pp 11455—11469

Inhibition of long non-coding RNA HOXA11-AS against neuroinflammation in Parkinson's disease model via targeting miR-124-3p mediated FSTL1/NF-κB axis

Expression characteristics of LncRNA HOXA11-AS and miR-124-3p in PD model. MPTP was used to induce a PD model in mice. (A) The neurological functions of PD mice were evaluated by spontaneous motor test, rotarod test and bevel test. (B, C) Immunohistochemistry was adopted to examine the number of TH and Caspase-3 positive cells and the microglia activation in SN area. (D) RT-PCR was conducted to determine the level of inflammatory factors IL-1β, IL-18, IL-6 and TNF-α in SN area. (E, F) Western blot was carried out to monitor the expression of FSTL1, NF-κB and NLRP3 inflammasome in SN area. (G, H) RT-PCR was adopted to examine the expression of HOXA11-AS and miR-124-3p in in SN area. (I) Person was applied to analyze the correlation between HOXA11-AS and miR-124-3p. ns P> 0.05, * P P P

Figure 1. Expression characteristics of LncRNA HOXA11-AS and miR-124-3p in PD model. MPTP was used to induce a PD model in mice. (A) The neurological functions of PD mice were evaluated by spontaneous motor test, rotarod test and bevel test. (B, C) Immunohistochemistry was adopted to examine the number of TH and Caspase-3 positive cells and the microglia activation in SN area. (D) RT-PCR was conducted to determine the level of inflammatory factors IL-1β, IL-18, IL-6 and TNF-α in SN area. (E, F) Western blot was carried out to monitor the expression of FSTL1, NF-κB and NLRP3 inflammasome in SN area. (G, H) RT-PCR was adopted to examine the expression of HOXA11-AS and miR-124-3p in in SN area. (I) Person was applied to analyze the correlation between HOXA11-AS and miR-124-3p. ns P> 0.05, * P <0.05, ** P <0.01, *** P <0.001 (vs. sham group).