Research Paper Volume 13, Issue 8 pp 11470—11490

Excessive expression of miR-1a by statin causes skeletal injury through targeting mitogen-activated protein kinase kinase kinase 1

Statin induces cell apoptosis via upregulation of miR-1a in skeletal muscle cells. (A–C) Cultured skeletal muscle cells were transfected with miR-1a mimics and inhibitors for 48 hours followed by statin treatment for 24 hours. Cells were subjected to detect the protein levels of pro-/cleaved-caspase3 in (A) bcl-2 and bax in (B) and pro-/cleaved-caspase7, 9 in (C) by western blot. N is 5 in each group. **P#P##PD, E) Cultured skeletal muscle cells were transfected with miR-1a inhibitor for 48 hours followed by statin treatment for 24 hours. Cell apoptosis was determined by TUNEL method in (D) and flow cytometry in (E). N is 5 in each group. **P##Ppost-hoc tests was used to determine P value in (A–E).

Figure 2. Statin induces cell apoptosis via upregulation of miR-1a in skeletal muscle cells. (AC) Cultured skeletal muscle cells were transfected with miR-1a mimics and inhibitors for 48 hours followed by statin treatment for 24 hours. Cells were subjected to detect the protein levels of pro-/cleaved-caspase3 in (A) bcl-2 and bax in (B) and pro-/cleaved-caspase7, 9 in (C) by western blot. N is 5 in each group. **P<0.01 vs. NC alone. #P<0.05 or ##P<0.01 vs. statin alone. (D, E) Cultured skeletal muscle cells were transfected with miR-1a inhibitor for 48 hours followed by statin treatment for 24 hours. Cell apoptosis was determined by TUNEL method in (D) and flow cytometry in (E). N is 5 in each group. **P<0.01 vs. NC alone. ##P<0.01 vs. statin alone. A one-way ANOVA followed by Tukey post-hoc tests was used to determine P value in (AE).