Research Paper Volume 13, Issue 8 pp 11470—11490

Excessive expression of miR-1a by statin causes skeletal injury through targeting mitogen-activated protein kinase kinase kinase 1

Statin induces cell apoptosis of skeletal muscle cells via downregulation of MAP3K1. Cultured skeletal muscle cells were transfected with plasmids MAP3K1 cDNA for 48 hours followed by statin treatment for 24 hours. Cells were subjected to detect the protein levels of pro-/cleaved-caspase3 in (A) bcl-2 and bax in (B) pro-/cleaved-caspase9 in (C) and pro-/cleaved-caspase7 in (D) by western blot. Cell apoptosis was determined by flow cytometry in (E). N is 5 in each group. **P##Ppost-hoc tests was used to determine P value in (A–E).

Figure 4. Statin induces cell apoptosis of skeletal muscle cells via downregulation of MAP3K1. Cultured skeletal muscle cells were transfected with plasmids MAP3K1 cDNA for 48 hours followed by statin treatment for 24 hours. Cells were subjected to detect the protein levels of pro-/cleaved-caspase3 in (A) bcl-2 and bax in (B) pro-/cleaved-caspase9 in (C) and pro-/cleaved-caspase7 in (D) by western blot. Cell apoptosis was determined by flow cytometry in (E). N is 5 in each group. **P<0.01 vs. vector alone. ##P<0.01 vs. statin plus vector. A one-way ANOVA followed by Tukey post-hoc tests was used to determine P value in (AE).