Research Paper Volume 13, Issue 8 pp 11528—11541

MicroRNA-532-5p protects against cerebral ischemia-reperfusion injury by directly targeting CXCL1

MiR-532-5p overexpression suppresses OGD/R-induced inflammation in SH-SY5Y cells via CXCL1/CXCR2/NF-κB axis. (A) Representative western blot shows the expression of CXCL1, CXCR2, p65 and p-p65 proteins in control or miR-532-5p agomir-transfected SH-SY5Y cells, treated with or without OGD/R. (B–C) The histogram plots show the expression levels of CXCL1 and CXCR2 proteins relative to β-actin protein levels in control or miR-532-5p agomir-transfected SH-SY5Y cells treated with or without OGD/R. (D) The histogram plot shows the levels of p-p65 relative to p65 levels in control or miR-532-5p agomir-transfected SH-SY5Y cells treated with or without OGD/R. (E) SH-SY5Y cells were transfected with NC or pcDNA3.1-CXCL1. The efficiency of cell transfection was detected by RT-qPCR. (F) The viability of SH-SY5Y cells was tested by CCK-8 assay. **P ##P ^^P

Figure 6. MiR-532-5p overexpression suppresses OGD/R-induced inflammation in SH-SY5Y cells via CXCL1/CXCR2/NF-κB axis. (A) Representative western blot shows the expression of CXCL1, CXCR2, p65 and p-p65 proteins in control or miR-532-5p agomir-transfected SH-SY5Y cells, treated with or without OGD/R. (BC) The histogram plots show the expression levels of CXCL1 and CXCR2 proteins relative to β-actin protein levels in control or miR-532-5p agomir-transfected SH-SY5Y cells treated with or without OGD/R. (D) The histogram plot shows the levels of p-p65 relative to p65 levels in control or miR-532-5p agomir-transfected SH-SY5Y cells treated with or without OGD/R. (E) SH-SY5Y cells were transfected with NC or pcDNA3.1-CXCL1. The efficiency of cell transfection was detected by RT-qPCR. (F) The viability of SH-SY5Y cells was tested by CCK-8 assay. **P < 0.01 compared to control; ##P < 0.01 compared to OGD/R. ^^P < 0.01 compared to OGD/R plus miR-532-5p agomir group. All experiments were performed in triplicate.