Research Paper Volume 13, Issue 8 pp 11528—11541

MicroRNA-532-5p protects against cerebral ischemia-reperfusion injury by directly targeting CXCL1

Overexpression of miR-532-5p reduces brain pathology in the in vivo CI/RI model rats. (A) RT-qPCR analysis shows the relative expression levels of miR-532-5p in the rat cerebral tissues in sham, miR-532-5p agomir, I/R and I/R plus miR-532-5p agomir groups. (B) Representative images show the brain tissues from sham, miR-532-5p agomir, I/R and I/R plus miR-532-5p agomir group rats. (C) Histogram plot shows cerebral infraction area based on TTC staining in sham, miR-532-5p agomir, I/R, and I/R plus miR-532-5p agomir group rats. **P D) The histogram plot shows cerebral water content in sham, miR-532-5p agomir, I/R and I/R plus miR-532-5p agomir group rats. (E, F) TUNEL staining results show the percentage of TUNEL-positive apoptotic neurons in the brain sections of sham, miR-532-5p agomir, I/R, and I/R plus miR-532-5p agomir group rats. **P #P ##P

Figure 7. Overexpression of miR-532-5p reduces brain pathology in the in vivo CI/RI model rats. (A) RT-qPCR analysis shows the relative expression levels of miR-532-5p in the rat cerebral tissues in sham, miR-532-5p agomir, I/R and I/R plus miR-532-5p agomir groups. (B) Representative images show the brain tissues from sham, miR-532-5p agomir, I/R and I/R plus miR-532-5p agomir group rats. (C) Histogram plot shows cerebral infraction area based on TTC staining in sham, miR-532-5p agomir, I/R, and I/R plus miR-532-5p agomir group rats. **P < 0.01 compared to sham. (D) The histogram plot shows cerebral water content in sham, miR-532-5p agomir, I/R and I/R plus miR-532-5p agomir group rats. (E, F) TUNEL staining results show the percentage of TUNEL-positive apoptotic neurons in the brain sections of sham, miR-532-5p agomir, I/R, and I/R plus miR-532-5p agomir group rats. **P < 0.01 compared to Sham; #P < 0.05, ##P < 0.01 compared to I/R. Each group consisted of 12 rats.