Research Paper Volume 13, Issue 7 pp 10703—10723

ASIC1 and ASIC3 mediate cellular senescence of human nucleus pulposus mesenchymal stem cells during intervertebral disc degeneration

Isolation and phenotyping of in vitro cultured NP-MSCs. Representative phase-contrast images of passage 1 (A) and passage 2 (3 weeks) (bar = 50 μm) (B) NP-MSCs showing adherent cells with a long-spindle shape morphology. (C) Analyses of cell surface markers of NP-MSCs by flow cytometry, showing positive CD105, CD90, CD73 and negative CD34, CD45 and HLA-DR. All the samples were from case 5.

Figure 1. Isolation and phenotyping of in vitro cultured NP-MSCs. Representative phase-contrast images of passage 1 (A) and passage 2 (3 weeks) (bar = 50 μm) (B) NP-MSCs showing adherent cells with a long-spindle shape morphology. (C) Analyses of cell surface markers of NP-MSCs by flow cytometry, showing positive CD105, CD90, CD73 and negative CD34, CD45 and HLA-DR. All the samples were from case 5.