Figure 1. Identification of hucMSC and hucMSC-ex. (A) Morphological identification of hucMSC. (B) Adipogenic differentiation of hucMSC. Adipogenic differentiation was analyzed by Oil-Red-O staining. (C) Osteogenic differentiation of hucMSC was shown by neutrophil alkaline phosphatase (NAP) staining (100x). (D) Flow cytometry analyses of phenotypic markers of hucMSC:CD19, CD29, CD90, CD105. (E) Representative AFM image of hucMSC-ex. (F) Representative TEM image of hucMSC-ex (Scale bar=200 nm). (G) The hucMSC-ex size distribution were analyzed using nanoparticle tracking analysis (NTA) with ZetaView_Particle Metrix. (H) Detection of hucMSC-ex surface marker expression by western blot.