Research Paper Volume 13, Issue 8 pp 11542—11563

HucMSC exosome-delivered 14-3-3ζ alleviates ultraviolet radiation-induced photodamage via SIRT1 pathway modulation

Knockdown the 14-3-3ζ inhibit SIRT1 expression in HaCaT and hucMSC cells. (A) Western blot analysis of SIRT1 expression level after HaCaT cells were transfected with containing the 14-3-3ζ sequence adenovirus expression vector. (B) Western blot analysis of SIRT1 expression level after HaCaT cells were transfected with containing the 14-3-3ζ sequence lentiviral expression vector. (C) Immunofluorescence detection of SIRT1 expression level after HaCaT cells were transfected with containing the 14-3-3ζ sequence lentiviral expression vector. (D) Western blot analysis of SIRT1 expression level after hucMSCs cells were transfected with containing the 14-3-3ζ sequence lentiviral expression vector. (E) Immunofluorescence detection of SIRT1 expression level after HaCaT cells were transfected with containing the 14-3-3ζ sequence lentiviral expression vector.

Figure 7. Knockdown the 14-3-3ζ inhibit SIRT1 expression in HaCaT and hucMSC cells. (A) Western blot analysis of SIRT1 expression level after HaCaT cells were transfected with containing the 14-3-3ζ sequence adenovirus expression vector. (B) Western blot analysis of SIRT1 expression level after HaCaT cells were transfected with containing the 14-3-3ζ sequence lentiviral expression vector. (C) Immunofluorescence detection of SIRT1 expression level after HaCaT cells were transfected with containing the 14-3-3ζ sequence lentiviral expression vector. (D) Western blot analysis of SIRT1 expression level after hucMSCs cells were transfected with containing the 14-3-3ζ sequence lentiviral expression vector. (E) Immunofluorescence detection of SIRT1 expression level after HaCaT cells were transfected with containing the 14-3-3ζ sequence lentiviral expression vector.