Research Paper Volume 13, Issue 6 pp 7998—8025

Evolution of mammalian longevity: age-related increase in autophagy in bats compared to other mammals

Basal and starvation-induced autophagy in skin-derived fibroblasts from P. kuhlii, M. myotis and M. musculus. (A) fold change of LC3 II/I ratio induced by serum withdrawal (p-values: two-tailed t-test; data represent mean ±SEM). Relationship between individual’s age and (B) starvation-induced LC3II/I fold change, (C) basal LC3 II/I ratio, (D) GAPDH normalized total LC3 signal. (B–D) Corresponding p-values indicate the significance of linear model and are indicated in the top right-hand corner of each plot. Models are plotted where significant. LQ – longevity quotient. Note that scales differ between species.

Figure 1. Basal and starvation-induced autophagy in skin-derived fibroblasts from P. kuhlii, M. myotis and M. musculus. (A) fold change of LC3 II/I ratio induced by serum withdrawal (p-values: two-tailed t-test; data represent mean ±SEM). Relationship between individual’s age and (B) starvation-induced LC3II/I fold change, (C) basal LC3 II/I ratio, (D) GAPDH normalized total LC3 signal. (BD) Corresponding p-values indicate the significance of linear model and are indicated in the top right-hand corner of each plot. Models are plotted where significant. LQ – longevity quotient. Note that scales differ between species.