Research Paper Volume 13, Issue 8 pp 11595—11609

Exosomes derived from mycobacterium tuberculosis-infected MSCs induce a pro-inflammatory response of macrophages

Pro-inflammatory response induced by Exo-MSCs-M.tb were time-dependent in macrophages. (A) The number of exosomes released from M.tb-infected MSCs at different time points post-injection. (B) Exosomal protein in M.tb-infected MSCs. at different time points post-injection. (C) TNF-α levels in macrophages treated with Exo-MSCs-M.tb (20μg) at different time points post-injection, as detected by ELISA assay 24 hours after exosome treatment. (D) The protein levels of 19-kDa lipoprotein, LAM, Hsp70, and MHC II in exosomes isolated from M.tb-infected MSCs at different time points post-injection, (E) The levels of phosphorylated IκBα or p38, and total p38 in macrophages treated with exosomes derived from MSCs or M.tb-infected MSCs, as detected by western blotting assay. *p 

Figure 5. Pro-inflammatory response induced by Exo-MSCs-M.tb were time-dependent in macrophages. (A) The number of exosomes released from M.tb-infected MSCs at different time points post-injection. (B) Exosomal protein in M.tb-infected MSCs. at different time points post-injection. (C) TNF-α levels in macrophages treated with Exo-MSCs-M.tb (20μg) at different time points post-injection, as detected by ELISA assay 24 hours after exosome treatment. (D) The protein levels of 19-kDa lipoprotein, LAM, Hsp70, and MHC II in exosomes isolated from M.tb-infected MSCs at different time points post-injection, (E) The levels of phosphorylated IκBα or p38, and total p38 in macrophages treated with exosomes derived from MSCs or M.tb-infected MSCs, as detected by western blotting assay. *p < 0.05.