Figure 2. Hsa_circ_0072309 was reversely looped and located in the tumor cell cytoplasm. (A) The diagram of hsa_circ_0072309. (B) Northern blot analyses of hsa_circ_0072309 with convergent primers and divergent primers. (C) The expression level of hsa_circ_0072309 and corresponding linear RNA with RNase R digestion. (D) The distribution of hsa_circ_0072309 in nuclear and cytoplasmic fractions in H1975 and H1650 cells. (E) RNA-FISH analysis of hsa_circ_0072309 in H1975 and H1650 cells. (F) ISH against hsa_circ_0072309 in human lung adenocarcinoma and adjacent normal tissues. Multiple t-test. N.S.: no significance; ***p < 0.001. Scale bars: 50 μm.