Figure 6. Hsa_circ_0072309 regulated miR-607 to modulate FTO expression in NSCLC. (A) RT-qPCR was performed to screen FTO as a miR-607 target gene. (B) Western blotting analyses of FTO protein levels with or without miR-607 mimic in HEK293 cells. Quantitative results were indicated in the right panel with GAPDH as internal reference. (C) RNA pulldown was performed to confirm the putative miR-607 binding sites with FTO 3'UTR. (D) A schematic diagram showing the putative miR-607 binding sites with FTO 3'-UTR. FTO 3'-UTR-WT and FTO 3'-UTR-Mut luciferase reporter vectors were cotransfected with miR-607 or miR-NC and then subjected to luciferase activity analyses. Quantitative results are indicated in the right panel. Student’s t-test (B). Two-way ANOVA (A, C–D). N.S.: no significance; **p < 0.01, ***p < 0.001.