Figure 3. Effects of SPRY4 overexpression on CRC cell apoptosis. The overexpression plasmid of SPRY4 was constructed and transfected into SW480 cells. Then, cell apoptotic rate was analyzed using flow cytometry assay (A, B). Furthermore, the protein expressions of Bcl-2, Bax, Cleaved caspase-3 and caspase-3 were measured using western blotting (C–F). **, ***p<0.01, 0.001 vs Oe-NC.