Figure 4. SPRY4 reduced the expression of EZH2 and MDM2. SW480 cells were transfected with overexpression plasmid of SPRY4 or co-treated with overexpression plasmid of SPRY4 and GSK126 (an EZH2 inhibitor). In each group, protein expressions of EZH2 and MDM2 were detected using western blotting (A–C). Immunoprecipitation assay, followed by western blotting, was used to evaluate the association between EZH2 and MDM2 (D, E). ***p<0.001 vs Control; ##p<0.01 vs Oe-NC.