Figure 6. The function of SPRY4 silence on apoptosis in SW480 cells. SW480 cells were treated with GSK126 or co-treated with GSK126 and si-SPRY4. Cell apoptotic rate was analyzed using flow cytometry assay (A, B). Moreover, the protein expressions of Bcl-2, Bax, Cleaved caspase-3 and caspase-3 were measured using western blotting (C–F). ***p<0.001 vs Control; ##, ###p<0.01, 0.001 vs GSK126.