Research Paper Volume 13, Issue 8 pp 11665—11677

SPRY4 suppresses proliferation and induces apoptosis of colorectal cancer cells by repressing oncogene EZH2

The function of SPRY4 silence on apoptosis in SW480 cells. SW480 cells were treated with GSK126 or co-treated with GSK126 and si-SPRY4. Cell apoptotic rate was analyzed using flow cytometry assay (A, B). Moreover, the protein expressions of Bcl-2, Bax, Cleaved caspase-3 and caspase-3 were measured using western blotting (C–F). ***p

Figure 6. The function of SPRY4 silence on apoptosis in SW480 cells. SW480 cells were treated with GSK126 or co-treated with GSK126 and si-SPRY4. Cell apoptotic rate was analyzed using flow cytometry assay (A, B). Moreover, the protein expressions of Bcl-2, Bax, Cleaved caspase-3 and caspase-3 were measured using western blotting (CF). ***p<0.001 vs Control; ##, ###p<0.01, 0.001 vs GSK126.