Research Paper Volume 13, Issue 8 pp 11665—11677

SPRY4 suppresses proliferation and induces apoptosis of colorectal cancer cells by repressing oncogene EZH2

SPRY4 regulated tumorigenesis in vivo. The SW480 cells transfected with Oe-SPRY4 or si-SPRY4 were subcutaneously implanted into the axilla of the nude mice. The weight and tumor volume of mice were monitored every four days (A, B). After sacrifice, the total tumors were separated from the mice and were weighed (C, D). The protein expression of EZH2 in tumor tissues was detected using western blot (E, F). The expression of Ki67 was analyzed by immunohistochemistry (G). This histology of liver, lung and kidney was measured by H&E staining (H). *, **, ***pSPRY4.

Figure 7. SPRY4 regulated tumorigenesis in vivo. The SW480 cells transfected with Oe-SPRY4 or si-SPRY4 were subcutaneously implanted into the axilla of the nude mice. The weight and tumor volume of mice were monitored every four days (A, B). After sacrifice, the total tumors were separated from the mice and were weighed (C, D). The protein expression of EZH2 in tumor tissues was detected using western blot (E, F). The expression of Ki67 was analyzed by immunohistochemistry (G). This histology of liver, lung and kidney was measured by H&E staining (H). *, **, ***p<0.05, 0.01, 0.001 vs Control; #, ###p<0.05, 0.001 vs si-SPRY4.