Research Paper Volume 13, Issue 8 pp 11705—11726

Selenomethionine protects hematopoietic stem/progenitor cells against cobalt nanoparticles by stimulating antioxidant actions and DNA repair functions

The protective effect of SeMet against CoNPs is partially associated to the improvement of anti-oxidant capacity. CD34+ HSC/HPCs were treated with 10 μM SeMet for 15 h and then subjected to 200 μM CoNPs for 24 h. Afterwards, cells underwent measurements of intracellular ROS level (A), T-AOC (B), GSH level (C) and GPx activity (D). *p  0.05, and ***p  0.001 vs. control cells that did no subjected to any treatments; #p  0.05, ##p  0.01 and ###p  0.001 vs. cells treated with CoNPs alone. CD34+ HSC/HPCs were treated with 10 μM SeMet alone or in combination with 1 μM H2O2 for 15 h. Cells were then treated with 200 μM CoNPs for 24 h, followed by measurements of cell viability (E) and apoptosis rate (F). ***p  0.001 vs. cells treated with CoNPs alone. #p  0.05 vs. cells treated with SeMet and CoNPs.

Figure 2. The protective effect of SeMet against CoNPs is partially associated to the improvement of anti-oxidant capacity. CD34+ HSC/HPCs were treated with 10 μM SeMet for 15 h and then subjected to 200 μM CoNPs for 24 h. Afterwards, cells underwent measurements of intracellular ROS level (A), T-AOC (B), GSH level (C) and GPx activity (D). *p < 0.05, and ***p < 0.001 vs. control cells that did no subjected to any treatments; #p < 0.05, ##p < 0.01 and ###p < 0.001 vs. cells treated with CoNPs alone. CD34+ HSC/HPCs were treated with 10 μM SeMet alone or in combination with 1 μM H2O2 for 15 h. Cells were then treated with 200 μM CoNPs for 24 h, followed by measurements of cell viability (E) and apoptosis rate (F). ***p < 0.001 vs. cells treated with CoNPs alone. #p < 0.05 vs. cells treated with SeMet and CoNPs.