Figure 5. The regulatory effects of CoNPs and SeMet on DNA damage response signal. CD34+ HSC/HPCs were treated with 10 μM SeMet and 200 μM CoNPs, alone or in combination, followed by western blot (A) and Co-IP assay (B). In Co-IP assay, HIF-1α, BRCA1 and cyclin B proteins were immunoprecipitated and the enrichment of ubiquitin in these proteins was further determined by western blot. *p < 0.05, **p < 0.01, and ***p < 0.001 vs. control cells that did no subjected to any treatments. #p < 0.05, ##p < 0.01 and ###p < 0.001 vs. cells treated with CoNPs alone.