Research Paper Volume 13, Issue 8 pp 11705—11726

Selenomethionine protects hematopoietic stem/progenitor cells against cobalt nanoparticles by stimulating antioxidant actions and DNA repair functions

HIF-1α is implicated in the toxic effect of CoNPs in CD34+ HSC/HPCs. This study used an activator of HIF-1α (DMOG) to imitate the activation of HIF-1α by CoNPs. Furthermore, HIF-1α was knocked down to determine whether HIF-1α partially mediates the toxic effect of CoNPs. After these treatments, cells underwent cell viability (A) and apoptosis (B) and western blot assays (C). *p  0.05, **p  0.01 and ***p  0.001 vs. control cells that did no subjected to any treatments. ##p  0.01 and ###p  0.001 vs. cells treated with CoNPs alone.

Figure 7. HIF-1α is implicated in the toxic effect of CoNPs in CD34+ HSC/HPCs. This study used an activator of HIF-1α (DMOG) to imitate the activation of HIF-1α by CoNPs. Furthermore, HIF-1α was knocked down to determine whether HIF-1α partially mediates the toxic effect of CoNPs. After these treatments, cells underwent cell viability (A) and apoptosis (B) and western blot assays (C). *p < 0.05, **p < 0.01 and ***p < 0.001 vs. control cells that did no subjected to any treatments. ##p < 0.01 and ###p < 0.001 vs. cells treated with CoNPs alone.