Research Paper Volume 13, Issue 8 pp 11752—11761

5-Lipoxygenase inhibition reduces inflammation and neuronal apoptosis via AKT signaling after subarachnoid hemorrhage in rats

Zileuton decreases 5-LOX expression through PI3K/AKT activation. (A) Immunofluorescent staining for 5-LOX (red) and NeuN (green) in the cortex of rats in the sham, SAH, SAH + Zileuton and SAH + Zileuton + LY294002 groups. Nuclei were counterstained with DAPI (blue). Final magnification, 400×; scale bars = 100 μm, n = 6 per group. (B–E) Representative images and quantitative analysis of 5-LOX and p-AKT after Zileuton treatment with and without LY294002. N = 6 in each group, *P &P #P

Figure 2. Zileuton decreases 5-LOX expression through PI3K/AKT activation. (A) Immunofluorescent staining for 5-LOX (red) and NeuN (green) in the cortex of rats in the sham, SAH, SAH + Zileuton and SAH + Zileuton + LY294002 groups. Nuclei were counterstained with DAPI (blue). Final magnification, 400×; scale bars = 100 μm, n = 6 per group. (BE) Representative images and quantitative analysis of 5-LOX and p-AKT after Zileuton treatment with and without LY294002. N = 6 in each group, *P < 0.05 vs sham group and &P < 0.05 vs SAH group, #P < 0.05 vs SAH + Zileuton group.