Research Paper Volume 13, Issue 8 pp 11762—11773

Characterization of murine mammary stem/progenitor cells in a D-galactose-induced aging model

D-galactose alters mammary stem/progenitor cell function in vitro. (A) Representative images showing in vitro serial passage of basal stem cell contained 3D organoids from primary organoids (P0) to P1 and P5 (scale bars, 100 μm); (B) Representative images showing in vitro serial passage of luminal progenitor cell contained 3D organoids from primary organoids (P0) to P1 and P5 (scale bars, 100 μm); (C) Quantification of the number of 3D organoids that can be passaged to P5 for stem/progenitor cells derived from control and D-galactose-treated mice (for each type of 3D organoids, 10 organoids per animal × 3 animals = 30 organoids were assayed).

Figure 4. D-galactose alters mammary stem/progenitor cell function in vitro. (A) Representative images showing in vitro serial passage of basal stem cell contained 3D organoids from primary organoids (P0) to P1 and P5 (scale bars, 100 μm); (B) Representative images showing in vitro serial passage of luminal progenitor cell contained 3D organoids from primary organoids (P0) to P1 and P5 (scale bars, 100 μm); (C) Quantification of the number of 3D organoids that can be passaged to P5 for stem/progenitor cells derived from control and D-galactose-treated mice (for each type of 3D organoids, 10 organoids per animal × 3 animals = 30 organoids were assayed).