Research Paper Volume 13, Issue 8 pp 11774—11785

Inhibition of sestrin 1 alleviates polycystic ovary syndrome by decreasing autophagy

Downregulation of sestrin 1 inhibited proliferation of KGN cells. KGN cells were transfected with siRNA control, sestrin 1 siRNA 1, sestrin 1 siRNA2, or sestrin 1 siRNA3 for 48 h. Sestrin 1 silencing efficiency was detected by (A) RT-qPCR (B) and western blot. The effect of sestrin 1 downregulation on proliferation of KGN cells was determined using (C) a CCK-8 assay and (D) EdU fluorescence staining. EdU positive cells were counted. *P **P

Figure 3. Downregulation of sestrin 1 inhibited proliferation of KGN cells. KGN cells were transfected with siRNA control, sestrin 1 siRNA 1, sestrin 1 siRNA2, or sestrin 1 siRNA3 for 48 h. Sestrin 1 silencing efficiency was detected by (A) RT-qPCR (B) and western blot. The effect of sestrin 1 downregulation on proliferation of KGN cells was determined using (C) a CCK-8 assay and (D) EdU fluorescence staining. EdU positive cells were counted. *P < 0.05, **P < 0.01 compared to the blank control.