Figure 5. Sestrin 1 knockdown increased oxidative stress in KGN cells by inhibiting autophagy. After treatment, (A) ROS level in KGN cells were measured using the DCFH-DA method. Results are presented as the percentage of blank control. (B–D) MDA, SOD, and GSH levels were measured by ELISA. **P < 0.01 compared to the blank control. #P < 0.05, ##P < 0.01 compared to the sestrin 1 siRNA2 group.