Research Paper Volume 13, Issue 8 pp 11774—11785

Inhibition of sestrin 1 alleviates polycystic ovary syndrome by decreasing autophagy

Sestrin 1 overexpression promoted proliferation of KGN cells by activating autophagy. KGN cells were transfected with sestrin 1 OE or vector-ctrl for 72 h respectively. (A) Sestrin 1 overexpression efficiency was evaluated by western blot. (B) Sestrin 1 expression was quantified. (C) The effect of sestrin 1 OE on proliferation of KGN cells was determined using a CCK-8 assay. (D) The effect of sestrin 1 OE on autophagy in KGN cells was determined using immunofluorescence staining for LC3. The percentage of LC3-positive cells indicates relative levels of autophagy. **P

Figure 7. Sestrin 1 overexpression promoted proliferation of KGN cells by activating autophagy. KGN cells were transfected with sestrin 1 OE or vector-ctrl for 72 h respectively. (A) Sestrin 1 overexpression efficiency was evaluated by western blot. (B) Sestrin 1 expression was quantified. (C) The effect of sestrin 1 OE on proliferation of KGN cells was determined using a CCK-8 assay. (D) The effect of sestrin 1 OE on autophagy in KGN cells was determined using immunofluorescence staining for LC3. The percentage of LC3-positive cells indicates relative levels of autophagy. **P < 0.01 compared to the blank control.