Research Paper Volume 13, Issue 10 pp 13571—13584

Long intergenic noncoding RNA 00665 promotes proliferation and inhibits apoptosis in colorectal cancer by regulating miR-126-5p

LINC00665 regulates PAK2 and FZD3 expression by competitively binding with miR-126-5p in CRC. (A) Predicted miR-126-5p binding sites in the PAK2 and FZD3 sequences. (B, C) Western blotting was used to detect the levels of PAK2 and FZD3 in cells transfected with the miR-126-5p mimic and inhibitor (n = 6; *P D) Luciferase assays in 293T cells transfected with miR-126-5p and wild-type or mutant PAK2 and the FZD3 target sequence (n = 6, *P E, F) Western blotting showed the expression of PAK2, FZD3 and β-catenin in cells transfected with si-LINC00665 or cotransfected with si-LINC00665 and miR-126-5p inhibitor (n = 6; *P P

Figure 6. LINC00665 regulates PAK2 and FZD3 expression by competitively binding with miR-126-5p in CRC. (A) Predicted miR-126-5p binding sites in the PAK2 and FZD3 sequences. (B, C) Western blotting was used to detect the levels of PAK2 and FZD3 in cells transfected with the miR-126-5p mimic and inhibitor (n = 6; *P < 0.05 vs NC). (D) Luciferase assays in 293T cells transfected with miR-126-5p and wild-type or mutant PAK2 and the FZD3 target sequence (n = 6, *P < 0.05 vs NC). (E, F) Western blotting showed the expression of PAK2, FZD3 and β-catenin in cells transfected with si-LINC00665 or cotransfected with si-LINC00665 and miR-126-5p inhibitor (n = 6; *P < 0.05 vs NC; #P < 0.05 vs si-LINC00665).