Research Paper Volume 13, Issue 8 pp 11808—11821

Exosomes derived from miR-1228 overexpressing bone marrow-mesenchymal stem cells promote growth of gastric cancer cells

Effect of exosomal-miR-1228 derived from BM-MSCs on growth and apoptosis of gastric cancer cells. (A) miR-1228 was lowly expressed in gastric cancer cells. (B) Expression of miR-1228 in gastric cancer cells transfected with miR-1228-mimics, miR-1228-inhibit, miR-NC after co-culture with exosomes. (C) After co-culture, the cells transfected with miR-1228-mimics had a slower proliferation, while those transfected with miR-1228-inhibit had an accelerated proliferation. (D) As shown by EdU cell proliferation assay, the proliferation of cells transfected miR-1228-mimics was accelerated, while that of transfected miR-1228-inhibit was slowed down. (E) After co-culture, the cells transfected with miR-1228-mimics had an accelerated apoptosis, while those transfected with miR-1228-inhibit had a decreased apoptosis. aPvs. miR-NC, bPvs. miR-NC.

Figure 5. Effect of exosomal-miR-1228 derived from BM-MSCs on growth and apoptosis of gastric cancer cells. (A) miR-1228 was lowly expressed in gastric cancer cells. (B) Expression of miR-1228 in gastric cancer cells transfected with miR-1228-mimics, miR-1228-inhibit, miR-NC after co-culture with exosomes. (C) After co-culture, the cells transfected with miR-1228-mimics had a slower proliferation, while those transfected with miR-1228-inhibit had an accelerated proliferation. (D) As shown by EdU cell proliferation assay, the proliferation of cells transfected miR-1228-mimics was accelerated, while that of transfected miR-1228-inhibit was slowed down. (E) After co-culture, the cells transfected with miR-1228-mimics had an accelerated apoptosis, while those transfected with miR-1228-inhibit had a decreased apoptosis. aP< 0.05 vs. miR-NC, bP< 0.01 vs. miR-NC.