Research Paper Volume 13, Issue 8 pp 11822—11832
ITPKA induces cell senescence, inhibits ovarian cancer tumorigenesis and can be downregulated by miR-203
Figure 1. ITPKA inhibited colony formation and induced cell senescence in ovarian cancer. (A) Overexpression of ITPKA in OVCAR3 and OVCA429 cells. Cells were transfected with pLVX plasmids or pLVX-Flag-ITPKA plasmids. After selection, the resistant cells were examined for the expression of Flag-tagged ITPKA. (B) Effects of ITPKA on the colony formation of OVCAR3 and OVCA429 cells were examined using a soft agar assay. (C) Statistical analysis of (B). (D) Effects of ITPKA on the senescence of OVCAR3 and OVCA429 cells were examined using β-gal staining. (E) Statistical analysis of (D). **P < 0.01.