Research Paper Volume 13, Issue 8 pp 11822—11832

ITPKA induces cell senescence, inhibits ovarian cancer tumorigenesis and can be downregulated by miR-203

ITPKA was downregulated in ovarian cancer and negatively regulated by miR-203. (A) IHC was performed to examine the protein levels in ovarian cancer and normal tissues. (B) GEPIA database mining was performed to determine the correlation between ITPKA expression and survival. (C) q-PCR was performed to examine the mRNA levels of ITPKA in ovarian cancer samples and normal tissues. (D) Western blotting was performed to examine the ITPKA protein level in ovarian cancer cell lines and normal ovarian epithelial cell lines (IOSE80 and IOSE144). (E) Illustration of miR-203 and ITPKA. (F) Effects of miR-203, mutant miR-203 and inhibitor on the expression of ITPKA were examined using q-PCR. (G) Soft agar assays were performed to examine the effects of ITPKA on the tumorigenicity of OVCAR3 cells. (H) Expression of miR-203 and ITPKA in ovarian cancer samples. **P

Figure 5. ITPKA was downregulated in ovarian cancer and negatively regulated by miR-203. (A) IHC was performed to examine the protein levels in ovarian cancer and normal tissues. (B) GEPIA database mining was performed to determine the correlation between ITPKA expression and survival. (C) q-PCR was performed to examine the mRNA levels of ITPKA in ovarian cancer samples and normal tissues. (D) Western blotting was performed to examine the ITPKA protein level in ovarian cancer cell lines and normal ovarian epithelial cell lines (IOSE80 and IOSE144). (E) Illustration of miR-203 and ITPKA. (F) Effects of miR-203, mutant miR-203 and inhibitor on the expression of ITPKA were examined using q-PCR. (G) Soft agar assays were performed to examine the effects of ITPKA on the tumorigenicity of OVCAR3 cells. (H) Expression of miR-203 and ITPKA in ovarian cancer samples. **P < 0.01.