Research Paper Volume 13, Issue 6 pp 7800—7827

Chronic treatment with acetaminophen protects against liver aging by targeting inflammation and oxidative stress

Analysis of markers of the M2 anti-inflammatory response and correlation of Cd8+ T cells with ALT. (A) mRNA expression of Arg1, Clec10a and Ccl2 in livers from 2 m/o, 14 m/o and 14 m/o + APAP mice, analyzed by q-PCR. Values were normalized against Hprt1 mRNA and expressed as fold of change vs. 2 m/o. (B) Linear regression between plasma ALT activity levels and the 2 populations of Cd8+ cells from 2 m/o, 14 m/o, and 14 m/o + APAP mice. Data are expressed as the mean ± S.E.M. (n = 6-16 (A), 9-12 (B) mice per group). Statistical analysis was performed by Kruskal-Wallis or one-way ANOVA test followed by their respective post-hoc test. ** P P vs. 2 m/o; #P ##P vs. 14 m/o.

Figure 5. Analysis of markers of the M2 anti-inflammatory response and correlation of Cd8+ T cells with ALT. (A) mRNA expression of Arg1, Clec10a and Ccl2 in livers from 2 m/o, 14 m/o and 14 m/o + APAP mice, analyzed by q-PCR. Values were normalized against Hprt1 mRNA and expressed as fold of change vs. 2 m/o. (B) Linear regression between plasma ALT activity levels and the 2 populations of Cd8+ cells from 2 m/o, 14 m/o, and 14 m/o + APAP mice. Data are expressed as the mean ± S.E.M. (n = 6-16 (A), 9-12 (B) mice per group). Statistical analysis was performed by Kruskal-Wallis or one-way ANOVA test followed by their respective post-hoc test. ** P < 0.01 and *** P < 0.001 vs. 2 m/o; #P < 0.05 and ##P < 0.01 vs. 14 m/o.