Research Paper Volume 13, Issue 7 pp 10749—10769

Dual activation of Hedgehog and Wnt/β-catenin signaling pathway caused by downregulation of SUFU targeted by miRNA-150 in human gastric cancer

SUFU is targeted by miRNA-150. (A) The regulation of SUFU by miRNA-150 was verified in HFE-145 and BGC-823 cells by western blotting. (B) The binding sites of miRNA-150 on the 3′UTR of SUFU mRNA were predicted. The sequencing result of a vector containing three mutated miRNA-150 binding sites is shown. (C) A SUFU 3′UTR luciferase reporter assay was performed in BGC-823 and HFE-145 cells co-transfected with miRNA-150 inhibitors or mimics, respectively, and the SUFU 3′UTR-WT or SUFU 3′UTR-MT reporter.

Figure 1. SUFU is targeted by miRNA-150. (A) The regulation of SUFU by miRNA-150 was verified in HFE-145 and BGC-823 cells by western blotting. (B) The binding sites of miRNA-150 on the 3′UTR of SUFU mRNA were predicted. The sequencing result of a vector containing three mutated miRNA-150 binding sites is shown. (C) A SUFU 3′UTR luciferase reporter assay was performed in BGC-823 and HFE-145 cells co-transfected with miRNA-150 inhibitors or mimics, respectively, and the SUFU 3′UTR-WT or SUFU 3′UTR-MT reporter.