Research Paper Volume 13, Issue 7 pp 10749—10769

Dual activation of Hedgehog and Wnt/β-catenin signaling pathway caused by downregulation of SUFU targeted by miRNA-150 in human gastric cancer

MiRNA-150 promotes tumor growth and activates Wnt and Hh signaling in vivo. (A) Tumor growth curves of volumes measured twice weekly after the injection of miRNA-150 inhibitors. Images of xenograft tumors and nude mice (N=6) and H&E stainings are shown. (B) Tumor weights after resection from nude mice. (C, D) The expression levels of miRNA-150 and Hh and Wnt downstream genes were reduced in vivo in tumors treated with miRNA-150 inhibitors. The expression of SUFU was increased in tumors treated with miRNA-150 inhibitors (NSC=6 tumors, 150-inh=3 tumors). Student’s t-test was performed. *p

Figure 5. MiRNA-150 promotes tumor growth and activates Wnt and Hh signaling in vivo. (A) Tumor growth curves of volumes measured twice weekly after the injection of miRNA-150 inhibitors. Images of xenograft tumors and nude mice (N=6) and H&E stainings are shown. (B) Tumor weights after resection from nude mice. (C, D) The expression levels of miRNA-150 and Hh and Wnt downstream genes were reduced in vivo in tumors treated with miRNA-150 inhibitors. The expression of SUFU was increased in tumors treated with miRNA-150 inhibitors (NSC=6 tumors, 150-inh=3 tumors). Student’s t-test was performed. *p<0.05.