Figure 3. hsa_circ_0110102 served as a sponge for miR-580-5p. (A) Schematic illustration of the predicted binding sites between hsa_circ_0110102 and seven candidate miRNAs. (B) RNA pull-down assay with hsa_circ_0110102 or oligo probes. (C) Relative levels of seven miRNAs in cell lysate pulled down by oligo or hsa_circ_0110102 probes were detected by RT-qPCR. (D) The predicted binding sites of miR-580-5p on hsa_circ_0110102. (E) Relative luciferase activity in Huh-7 and HepG2 cells after transfection of hsa_circ_0110102 WT/Mut and 3′-UTR of miR-580-5p. (F) Anti-AGO2 RIP was performed in Huh-7 and HepG2 cells after miR-580-5p NC or mimic transfection. Data are presented as mean ± standard error. *, P < 0.05.