Figure 4. miR-580-5p was overexpressed in HCC tumor tissues and cell lines. (A) miR-580-5p levels in 49 normal and 369 primary HCC tissues. (B) Kaplan–Meier survival curve of patients in low- and high-expression miR-580-5p groups. (C) miR-580-5p levels in five HCC cell lines and LO2 cells were examined by RT-PCR. Huh-7 and HepG2 cells were transfected with miR-580-5p NC or mimic for 48 h. (D) The miR-580-5p levels were examined with RT-qPCR. (E) Cell viability was detected using the CCK-8 assay. (F and G) EdU assays were used to detect the DNA synthesis in Huh-7 and HepG2 cells. (H) Cell cycle was detected using flow cytometry assays. Data are presented as mean ± standard error. *, P < 0.05.