Research Paper Volume 13, Issue 9 pp 12466—12478

Supplementary research on K150del variant of activated protein C

Cytoprotective effects of APC derivatives in response to staurosporine-induced apoptosis. (A) The anti-apoptotic activity of APC-WT and APC-DEL in HUVECs in response to staurosporine was evaluated using the Annexin V–FITC apoptosis detection kit. The everted phosphatidylserine in cell membranes, probed by Annexin V labeled with FITC, reflected early apoptosis (Q2), whereas the exposed cell nuclei, probed by propidium iodide, reflected middle and late apoptosis (Q3). The total apoptosis rate was equal to Q2 plus Q3. (B) Statistical analysis of data derived from three independent measurements shown in (A).

Figure 6. Cytoprotective effects of APC derivatives in response to staurosporine-induced apoptosis. (A) The anti-apoptotic activity of APC-WT and APC-DEL in HUVECs in response to staurosporine was evaluated using the Annexin V–FITC apoptosis detection kit. The everted phosphatidylserine in cell membranes, probed by Annexin V labeled with FITC, reflected early apoptosis (Q2), whereas the exposed cell nuclei, probed by propidium iodide, reflected middle and late apoptosis (Q3). The total apoptosis rate was equal to Q2 plus Q3. (B) Statistical analysis of data derived from three independent measurements shown in (A).