Research Paper Volume 13, Issue 8 pp 12007—12015

LINC00514 promotes gastric cancer cell growth and EMT progression via miR-204-3p/KRAS

Elevated expression of LINC00514 enhanced cell growth and EMT progression by sponging KRAS. (A) The level of KRAS was downregulated in HGC-27 cells after transfection with si-KRAS vectors. (B) The expression of KRAS was measured by qRT-PCR. (C) Downregulation of KRAS suppressed CDK2 expression in LINC00514-overexpressing HGC-27 cells. (D) Knockdown of KRAS inhibited cell growth in LINC00514-overexpressing HGC-27 cell. (E) The expression levels of E-cadherin, N-cadherin and vimentin were detected by qRT-PCR. *p

Figure 5. Elevated expression of LINC00514 enhanced cell growth and EMT progression by sponging KRAS. (A) The level of KRAS was downregulated in HGC-27 cells after transfection with si-KRAS vectors. (B) The expression of KRAS was measured by qRT-PCR. (C) Downregulation of KRAS suppressed CDK2 expression in LINC00514-overexpressing HGC-27 cells. (D) Knockdown of KRAS inhibited cell growth in LINC00514-overexpressing HGC-27 cell. (E) The expression levels of E-cadherin, N-cadherin and vimentin were detected by qRT-PCR. *p<0.05, **p<0.01 and ***p<0.001.